Reticulocyte counting using flow cytometry.
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چکیده
منابع مشابه
Reticulocyte counting using flow cytometry.
A flow cytometric method for the quantitation of reticulocytes was refined for routine laboratory use. Blood (2 microliters) is added to 2 ml of 0.4 microM thiazole orange in phosphate buffered saline, incubated at room temperature for 90 minutes, and analysed on a Coulter EPICS Profile flow cytometer, with gating for red cells on the basis of forward and right angled light scatter. Blood (2 mi...
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Background: Reticulocytes are immature red blood cells with RNA, spending the final stages of their maturation in the peripheral blood. The number of reticulocytes in the peripheral blood is the salient evidence of the effectiveness of bone marrow to produce red blood cells. Currently, reticulocyte count is done manually or automatically in clinical laboratories. Difficulties and limitations of...
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The reticulocyte count (RC) is a key diagnostic test in the evaluation, classification, and response to therapy of anemia. The RC, as determined by manual methods, has a frustrating inherent imprecision owing to its binomial counting statistics (i.e., low counts/low precision) and inaccuracy because of inter- and intraobserver variability as to what indeed is a reticulocyte. Fluorescent activat...
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Introduction Bone marrow examination is essential procedure for evaluation of a variety of clinical hematologic disorders and for preclinical toxicity studies of anticancer compounds and immunosuppressive agents [1,2]. This evaluation has been classically performed by morphologic assessment of bone marrow aspiration smears and core biopsy specimens by use of light microscopy. Differential cell ...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1990
ISSN: 0021-9746
DOI: 10.1136/jcp.43.8.675